Bortezomib enhances the sensitivity of prostate cancer cells to natural killer cell-mediated cytotoxicity / 中华男科学杂志

<p><b>OBJECTIVE</b>To investigate whether bortezomib can enhance the sensitivity of human prostate cancer (PCa) cells to natural killer (NK) cell-mediated cytotoxicity, and whether it produces the same effect on different PCa cell lines.</p><p><b>METHODS</b>We treated androgen-dependent PCa LNCaP cells and androgen-independent PCa DU145 cells with bortezomib at the concentrations of 0, 5, 10, 15, 20 and 25 nmol/L for 24, 48 and 72 hours, and then detected the proliferation and apoptosis of the tumor cells by CCK-8 and Annexin V/PI, respectively.</p><p><b>RESULTS</b>The proliferation rates of the DU145 cells treated with 15, 20 and 25 nmol/L bortezomib were (82.79 +/-2.04)%, (73.59+/- 2.95)% and (74.16+/- 6. 16)% at 48 hours and (71.24+/- 5.30)%, (51.20+/- 2.91)% and (38.02+/- 2.67)% at 72 hours, and those of the LNCaP cells were (77.04+/- 7.74)% , (42.61 +/- 6.62)% and (23.85 +/-6.04)% at 48 hours and (36.45 +/-7.02)%, (14.94 +/-5.76)% and (11.65 +/-5. 87)% at 72 hours, both significantly inhibited as compared with the control group (P <0.05). At 24 hours, the apoptosis rates of the DU145 cells treated with 15, 20 and 25 nmol/L bortezomib were (14.41 +/- 1.32)% , (16.13 +/- 1.55)% and (14.48 +/- 1.42)% , and those of the LNCaP cells treated with 20 and 25 nmol/L bortezomib were (12.77 +/- 1.28)% and (14. 84 +/- 1.65)% , significantly higher than those of the control group (P <0.05) , and the DU145 cells showed an even higher sensitivity to bortezomib than the LNCaP cells. Bortezomib failed to sensitize these two cell lines to NK cell-mediated cytotoxicity in short-term assay, while long-term assay manifested that the apoptosis rates of DU145 and LNCaP cells after treated with 20 nmol/L bortezomib + NK cells were (41.83 +/- 5.06)% and (30.31 +/- 3.62)% , respectively, significantly higher</p><p><b>CONCLUSION</b>Bortezomib enhances the sensitivity of than those after treated with either bortezomib or NK cells alone (P <0.05). PCa cells to NK cell-mediated cytotoxicity and adds to the effect of current cancer therapies, and it is more efficacious for androgen-independent prostate cancer.</p>

An experimental study of the effect of burying testis in thigh pocket on spermatogenesis / 中华整形外科杂志

<p><b>OBJECTIVE</b>To explore the effect of burying testis in thigh pocket on spermatogenesis.</p><p><b>METHODS</b>Guizhou miniature male pigs at child-bearing period were randomly divided to receive operation of scrotum incision and dissection with the testis burying in thigh pocket (experimental group) or without (control group). 3 months later, testis biopsy was performed on 2 pigs from each group for pathological examination. Then every male pig from both experimental (n = 6) and control group (n = 6) got a mating partner and lived together for 3 months. The fertility of the male pigs was observed. 6 months after operation, testis biopsy was performed again on all the animals from both the groups.</p><p><b>RESULTS</b>Both at 3 months and 6 months after operation, the pathological examination showed the spermatogenic cells of all stage in contorted seminiferous tubules markedly decreased with no mature sperm in experimental group, while normal spermatogenic cells with mature sperm in control group. After the male pigs lived with mating partners for 3 months, no female pigs staying with the experimental group became pregnant, but the male pigs in control group had a normal fertility.</p><p><b>CONCLUSIONS</b>Burying testis in thigh pocket impedes spermatogenesis in the miniature male pig. So burying testis in thigh pocket is not recommended for patients with scrotum skin defect who wish to remain fertile.</p>

An experiment study and clinical observation of the testicle spermatogenesis after scrotum reconstruction / 中华整形外科杂志

<p><b>OBJECTIVE</b>To explore the effect of scrotum reconstruction with a skin flap on spermatogenesis.</p><p><b>METHODS</b>Two patients who underwent scrotum reconstruction with the skin flap were followed up for four years. Their sperm quality, sex function, sexual hormone, and testis biopsy were examined. To exclude the influential factors of testis and spermatic cord contusing, an experiment study was designed and performed in rabbits. The scrotal skin of the rabbits was stripped off and the scrotum was reconstructed with a hypogastric skin flap.</p><p><b>RESULTS</b>The clinical follow-up indicated that in the early postoperative period, the reconstruction did not impede spermatogenesis, but the arrest of spermatogenesis happened with time. The experimental results showed that the sperm count of the rabbits decreased obviously and the rabbits became sterile two months after scrotum reconstruction.</p><p><b>CONCLUSION</b>The thick skin flap is not recommended for scrotum reconstruction.</p>